Baculovirus as versatile vectors for protein expression in insect and mammalian cells

被引：717

作者：

Kost, TA

Condreay, JP

Jarvis, DL

机构：

[1] GlaxoSmithKline R&D, Gene Express Prot Biochem, Res Triangle Pk, NC 27709 USA

[2] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA

来源：

NATURE BIOTECHNOLOGY | 2005年 / 23卷 / 05期

关键词：

D O I：

10.1038/nbt1095

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Today, many thousands of recombinant proteins, ranging from cytosolic enzymes to membrane-bound proteins, have been successfully produced in baculovirus-infected insect cells. Yet, in addition to its value in producing recombinant proteins in insect cells and larvae, this viral vector system continues to evolve in new and unexpected ways. This is exemplified by the development of engineered insect cell lines to mimic mammalian cell glycosylation of expressed proteins, baculovirus display strategies and the application of the virus as a mammalian-cell gene delivery vector. Novel vector design and cell engineering approaches will serve to further enhance the value of baculovirus technology.

引用

收藏

页码：567 / 575

页数：9

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