Mouse MTH2 protein which prevents mutations caused by 8-oxoguanine nucleotides

被引：97

作者：

Cai, JP

Ishibashi, T

Takagi, Y

Hayakawa, H

Sekiguchi, M

机构：

[1] Fukuoka Dent Coll, Dept Biol, Fukuoka 8140193, Japan

[2] Fukuoka Dent Coll, Frontier Res Ctr, Fukuoka 8140193, Japan

[3] Kyushu Univ, Grad Sch Med Sci, Dept Med Biochem, Fukuoka 8128582, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 305卷 / 04期

关键词：

oxygen radical; mutation; MutT; 8-oxoguanine; MTH2;

D O I：

10.1016/S0006-291X(03)00864-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MutT-related proteins degrade 8-oxo-7,8-dihydrodeoxyguanosine triphosphate (8-oxo-dGTP), a mutagenic substrate for DNA synthesis, in the nucleotide pool, thereby preventing DNA replication errors. During a search of GenBank EST database, we found a new member of MutT-related protein, MTH2, which possesses the 23-amino acid MutT module. The cloned mouse MTH2 (mMTH2) cDNA was expressed in Escherichia coli mutT(-) cells and the protein was purified. mMTH2 protein hydrolyzes 8-oxo-dGTP to 8-oxo-dGMP. with Kin of 32 muM. Expression of cDNA for mMTH2 reduced significantly the elevated level of spontaneous mutation frequency of E. coli mutT- cells. Thus, MTH2 has a potential to protect the genetic material from the untoward effects of endogenous oxygen radicals. MTH2 could act as an MTH1 redundancy factor. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：1073 / 1077

页数：5

共 22 条

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