Isolation of up- and down-regulated cDNAs associated with hepatocellular carcinoma by a subtraction-enhanced display technique

被引:21
作者
Wu, CG
Hakvoort, TBM
Lamers, WH
Chamuleau, RAFM
机构
[1] UNIV AMSTERDAM, ACAD MED CTR, DEPT ANAT & EMBRYOL, 1105 AZ AMSTERDAM, NETHERLANDS
[2] UNIV AMSTERDAM, ACAD MED CTR, DEPT EXPTL INTERNAL MED, 1105 AZ AMSTERDAM, NETHERLANDS
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 1996年 / 1315卷 / 03期
关键词
hepatocellular-carcinoma; tumor marker; Gel display; subtractive-hybridization;
D O I
10.1016/0925-4439(95)00117-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Identification of gene products exclusively or abundantly expressed in hepatocellular carcinoma (HCC) and in normal Liver may yield novel tumor markers. We have isolated 36 up- and down-regulated cDNAs from diethylnitrosamine-induced rat hepatocellular carcinoma and normal liver tissue by using the subtraction-enhanced display technique. Nucleotide sequence analysis revealed that the majority of 20 subtraction-enriched cDNA fragments were well-characterized oncogenes and tumor-associated genes, like c-myc, alpha-prothymosin, p21, glutathione-S transferase (G-ST) and al-acid glycoprotein (AGP). As demonstrated by Northern blot detection, all of them were preferentially expressed either in HCC or in normal liver (2- to 7-fold). As paradigm, G-ST and AGP were shown to be exclusively overexpressed in tumor nodules by in situ hybridization. In addition, 14 of the remaining 16 novel genes were analysed on Northern blot, 10 of which were differentially expressed in HCC.
引用
收藏
页码:169 / 175
页数:7
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