pH-Degradable Mannosylated Nanogels for Dendritic Cell Targeting

被引：68

作者：

De Coen, Ruben ^{[1
]}

Vanparijs, Nane ^{[1
]}

Risseeuw, Martijn D. P. ^{[1
]}

Lybaert, Lien ^{[1
]}

Louage, Benoit ^{[1
]}

De Koker, Stefaan ^{[2
]}

Kumar, Vimal ^{[2
]}

Grooten, Johan ^{[2
]}

Taylor, Leeanne ^{[3
]}

Ayres, Neil ^{[3
]}

Van Calenbergh, Serge ^{[1
]}

Nuhn, Lutz ^{[1
]}

De Geest, Bruno G. ^{[1
]}

机构：

[1] Univ Ghent, Dept Pharmaceut, B-9000 Ghent, Belgium

[2] Univ Ghent, Dept Biomed Mol Biol, B-9000 Ghent, Belgium

[3] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA

来源：

BIOMACROMOLECULES | 2016年 / 17卷 / 07期

关键词：

MANNOSE RECEPTOR; RAFT POLYMERIZATION; SYNTHETIC VACCINES; DC-SIGN; POLYMERS; IMMUNITY; LECTIN; NANOPARTICLES; GLYCOPOLYMERS; ACTIVATION;

D O I：

10.1021/acs.biomac.6b00685

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the design of glycosylated nanogels via core-cross linking of amphiphilic non-water-soluble block copolymers composed of an acetylated glycosylated block and a pentafluorophenyl (PFP) activated ester block prepared by reversible addition fragmentation (RAFT) polymerization. Self-assembly, pH-sensitive core-cross-linking, and removal of remaining PFP esters and protecting groups are achieved in one pot and yield fully hydrated sub-100 nm nanogels. Using cell subsets that exhibit high and low expression of the mannose receptor (MR) under conditions that suppress active endocytosis, we show that mannosylated but not galactosylated nanogels can efficiently target the MR that is expressed on the cell surface of primary dendritic cells (DCs). These nanogels hold promise for immunological applications involving DCs and macrophage subsets.

引用

页码：2479 / 2488

页数：10

共 61 条

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