Electron cryomicroscopy methods

被引:29
作者
Unger, VM [1 ]
机构
[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
关键词
D O I
10.1016/S0959-440X(00)00260-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electron cryomicroscopy methods comprise a rapidly expanding field providing insights into the structure and function of biological macromolecules and their supramolecular assemblies. The 3.8 Angstrom resolution structure of the membrane protein aquaporin, a view of the herpesvirus capsid at 8.5 Angstrom and the 10 Angstrom resolution structure of the spliceosomal U1 small nuclear ribonucleoprotein complex are three outstanding examples emphasizing the versatility of this technique.
引用
收藏
页码:548 / 554
页数:7
相关论文
共 55 条
[1]   Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle [J].
Agrawal, RK ;
Spahn, CMT ;
Penczek, P ;
Grassucci, RA ;
Nierhaus, KH ;
Frank, J .
JOURNAL OF CELL BIOLOGY, 2000, 150 (03) :447-459
[2]   Protein crystallization on lipid layers and structure determination of the RNA polymerase II transcription initiation complex [J].
Asturias, FJ ;
Kornberg, RD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (11) :6813-6816
[3]   Toward detecting and identifying macromolecules in a cellular context:: Template matching applied to electron tomograms [J].
Böhm, J ;
Frangakis, AS ;
Hegerl, R ;
Nickell, S ;
Typke, D ;
Baumeister, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) :14245-14250
[4]   Leginon: An automated system for acquisition of images from vitreous ice specimens [J].
Carragher, B ;
Kisseberth, N ;
Kriegman, D ;
Milligan, RA ;
Potter, CS ;
Pulokas, J ;
Reilein, A .
JOURNAL OF STRUCTURAL BIOLOGY, 2000, 132 (01) :33-45
[5]   Projection structure and oligomeric properties of a bacterial core protein translocase [J].
Collinson, I ;
Breyton, C ;
Duong, F ;
Tziatzios, C ;
Schubert, D ;
Or, E ;
Rapoport, T ;
Kühlbrandt, W .
EMBO JOURNAL, 2001, 20 (10) :2462-2471
[6]   Projection structure of a plant vacuole membrane aquaporin by electron cryo-crystallography [J].
Daniels, MJ ;
Chrispeels, MJ ;
Yeager, M .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 294 (05) :1337-1349
[7]   SPOT-SCAN IMAGING IN TRANSMISSION ELECTRON-MICROSCOPY [J].
DOWNING, KH .
SCIENCE, 1991, 251 (4989) :53-59
[8]   AUTOMATIC FOCUS CORRECTION FOR SPOT-SCAN IMAGING OF TILTED SPECIMENS [J].
DOWNING, KH .
ULTRAMICROSCOPY, 1992, 46 (1-4) :199-206
[9]   Structure analysis of soluble proteins using electron crystallography [J].
Ellis, MJ ;
Hebert, H .
MICRON, 2001, 32 (05) :541-550
[10]   Perforated support foils with pre-defined hole size, shape and arrangement [J].
Ermantraut, E ;
Wohlfart, K ;
Tichelaar, W .
ULTRAMICROSCOPY, 1998, 74 (1-2) :75-81