Genetic studies of the role of fatty acid and coenzyme A in photocatalytic inactivation of Escherichia coli

被引：54

作者：

Gao, Minghui ^{[1
]}

An, Taicheng ^{[2
]}

Li, Guiying ^{[2
]}

Nie, Xin ^{[2
]}

Yip, Ho-Yin ^{[1
]}

Zhao, Huijun ^{[3
,4
]}

Wong, Po-Keung ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China

[2] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China

[3] Griffith Univ, Griffith Sch Environm, Nathan, Qld 4222, Australia

[4] Griffith Univ, Ctr Clean Environm & Energy, Nathan, Qld 4222, Australia

来源：

WATER RESEARCH | 2012年 / 46卷 / 13期

基金：

美国国家科学基金会;

关键词：

Photocatalytic inactivation; Single-gene deletion mutants; Fatty acid composition; Coenzyme A; TIO2; PHOTOCATALYSIS; VISIBLE-LIGHT; BACTERIAL DISINFECTION; MICROBIAL-CELLS; ATR-FTIR; MEMBRANE; STERILIZATION; DEGRADATION; TEMPERATURE; ADAPTATION;

D O I：

10.1016/j.watres.2012.05.033

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

The roles of bacterial cellular components, namely, fatty acid profile and coenzyme A, in photocatalytic inactivation of bacteria were investigated. Escherichia coli BW25113, as a "parental strain", and its isogenic single-gene deletion mutants E. coli JW1081 (fabF(-) mutant) and E. coli JW3942 (coaA(-) mutant) showed different susceptibilities towards photocatalytic inactivation by titanium dioxide (TiO2, irradiated by UVA lamps (lambda = 365 nm)). Regulating the fatty acid composition through pre-incubation temperature adjustment demonstrated the crucial role of cell membrane fatty acid profile in bacterial susceptibility towards photocatalytic inactivation, while the lower coenzyme A level in coaA(-) mutant correlated well with its lower susceptibility towards photocatalytic inactivation. Furthermore, transmission electron microscopic study demonstrated the photocatalytic destruction process of bacterial cells. This is the first study using single-gene deletion mutants to explore better understanding of the photocatalytic inactivation mechanism of E. coli. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：3951 / 3957

页数：7

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