Application of reinforced frontal affinity chromatography and advanced processing procedure to the study of the binding property of a Caenorhabditis elegans galectin

被引：37

作者：

Arata, Y ^{[1
]}

Hirabayashi, J ^{[1
]}

Kasai, K ^{[1
]}

机构：

[1] Teikyo Univ, Fac Pharmaceut Sci, Dept Biol Chem, Sagamiko, Kanagawa 1990195, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 905卷 / 1-2期

关键词：

frontal affinity chromatography; Caenorhabditis elegans; galectin; proteins; lectins;

D O I：

10.1016/S0021-9673(00)00967-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Frontal affinity chromatography is a very useful and simple method to analyze molecular interactions between an analyte and an immobilized ligand by calculating the extent of "retardation" of the elution front. We developed a very simple and efficient data-processing procedure that enables the measurement of very small differences in retardation with precision. This procedure was successfully applied to comparison of the binding properties of recombinant C. elegans galectins for their ligand. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：337 / 343

页数：7

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