Effects of high-fat, low-cholesterol diets on hepatic lipid peroxidation and antioxidants in apolipoprotein E-deficient mice

被引:30
作者
Ferré, N
Camps, J
Paul, A
Cabré, M
Calleja, L
Osada, J
Joven, J
机构
[1] Hosp Univ Sant Joan, Ctr Recerca Biomed, Reus 43201, Catalunya, Spain
[2] Univ Rovira & Virgili, Fac Med, Unitat Bioquim, Tarragona, Spain
[3] Univ Zaragoza, Fac Vet, Dept Bioquim & Biol Mol & Celular, E-50009 Zaragoza, Spain
关键词
atherosclerosis; dietary fat; lipid peroxidation; vitamin E;
D O I
10.1023/A:1007296919243
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The present study describes the effects of several high-fat low-cholesterol antiatherogenic diets on the hepatic lipid peroxidation and hepatic antioxidant systems in apolipoprotein E-deficient mice. Eighty mice were distributed into five groups and fed with regular mouse chow or chow supplemented with coconut, palm, olive and sunflower seed oils. After ten weeks, they were sacrificed and the livers were removed so that lipid peroxidation and alpha -tocopherol concentrations, and superoxide dismutase, glutathione peroxidase and glutathione reductase activities could be measured. The size of the atherosclerotic lesions in the aortas was also measured. Results showed that the diets supplemented with olive oil, palm oil or sunflower seed oil significantly decreased the size of the lesion. However, there was an association between those mice that were on diets supplemented with palm or coconut oils and a significant increase in hepatic lipid peroxidation. This association was not found in animals fed with olive or sunflower seed oils, the diets with the highest content of vitamin E. The dietary content of vitamin E was significantly correlated (r = 0.98; p < 0.05) with the hepatic concentration of this compound. Our study suggests that the high content of vitamin E in olive oil or sunflower seed oil may protect from the undesirable hepatotoxic effects of high-fat diets in apo E-deficient mice and that this should be taken into account when these diets are used to prevent atherosclerosis.
引用
收藏
页码:165 / 169
页数:5
相关论文
共 32 条
  • [1] CABRE M, 1995, INT J VITAM NUTR RES, V65, P45
  • [2] Low-cholesterol and high-fat diets reduce atherosclerotic lesion development in ApoE-knockout mice
    Calleja, L
    París, MA
    Paul, A
    Vilella, E
    Joven, J
    Jiménez, A
    Beltrán, G
    Uceda, M
    Maeda, N
    Osada, J
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1999, 19 (10) : 2368 - 2375
  • [3] CYTOTOXICITY AND GENOTOXICITY OF LIPID-OXIDATION PRODUCTS
    ESTERBAUER, H
    [J]. AMERICAN JOURNAL OF CLINICAL NUTRITION, 1993, 57 (05) : 779 - 786
  • [4] Inhibition instead of enhancement of lipid peroxidation by pretreatment with the carcinogenic peroxisome proliferator nafenopin in rat liver exposed to a high single dose of corn oil
    Huber, WW
    GraslKraupp, B
    Stekel, H
    Gschwentner, C
    Lang, H
    SchulteHermann, R
    [J]. ARCHIVES OF TOXICOLOGY, 1997, 71 (09) : 575 - 581
  • [5] Kris-Etherton PM, 1999, AM J CLIN NUTR, V70, P1009
  • [6] Lake B. G., 1987, BIOCH TOXICOLOGY PRA, P183
  • [7] Role of mitochondria in oxidative stress and ageing
    Lenaz, G
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1366 (1-2): : 53 - 67
  • [8] Dietary vegetable oils and alpha-tocopherol reduce lipid oxidation in rabbit muscle
    LopezBote, CJ
    Rey, AI
    Sanz, M
    Gray, JI
    Buckley, DJ
    [J]. JOURNAL OF NUTRITION, 1997, 127 (06) : 1176 - 1182
  • [9] Monounsaturated fatty acid-enriched diet decreases plasma plasminogen activator inhibitor type 1
    LopezSegura, F
    Velasco, F
    LopezMiranda, J
    Castro, P
    LopezPedrera, R
    Blanco, A
    JimenezPereperez, J
    Torres, A
    Trujillo, J
    Ordovas, JM
    PerezJimenez, F
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1996, 16 (01) : 82 - 88
  • [10] Plasma LDL oxidation leads to its aggregation in the atherosclerotic apolipoprotein E-deficient mice
    Maor, I
    Hayek, T
    Coleman, R
    Aviram, M
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1997, 17 (11) : 2995 - 3005