Rapid detection of poly(ADP-ribose) polymerase by enzyme-linked immunosorbent assay during its purification and improvement of its purification

被引：2

作者：

Sallmann, FR

Plancke, YD

Poirier, GG

机构：

[1] CHUQ, CHUL, Res Ctr, Hlth & Environm Unit,PolyADP Ribose Metab Grp, Ste Foy, PQ G1V 4G2, Canada

[2] Univ Lille, CNRS, UMR 111, Chim Biol Lab, Villeneuve Dascq, France

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1998年 / 185卷 / 1-2期

关键词：

poly(ADP-ribose) polymerase; enzyme-linked immunosorbent assay; purification; Heparin Sepharose;

D O I：

10.1023/A:1006861015142

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report a new detection method for the purification of poly(ADP-ribose) polymerase (PARP). PARP purification generates many fractions in which PARP is usually detected by a time consuming activity assay. The development of a new method was also needed in order to decrease the utilization of radioactivity. This new method, based on an enzyme-linked immunosorbent assay (ELISA), is very rapid, sensitive, and avoids most radioactivity. Moreover, to illustrate this method, a new matrix was used, the Heparin Sepharose. This matrix was chosen for its affinity for the DNA binding proteins and because it allows the separation of whole PARP from its proteolytic fragments.

引用

页码：199 / 203

页数：5

共 9 条

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