The binding site of the IclR repressor protein overlaps the promoter of aceBAK

被引：29

作者：

Pan, B ^{[1
]}

Unnikrishnan, I ^{[1
]}

LaPorte, DC ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT BIOCHEM,MINNEAPOLIS,MN 55455

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 13期

关键词：

D O I：

10.1128/jb.178.13.3982-3984.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In Escherichia coli, repression of the aceBAK operon is mediated by the IcIR protein. We used an in vitro of oligonucleotide selection technique to determine the consensus recognition sequence for IcIR, Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of the promoter.

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页码：3982 / 3984

页数：3

相关论文

共 24 条

[1] THE PHOSPHORYLATION OF ESCHERICHIA-COLI ISOCITRATE DEHYDROGENASE IN INTACT-CELLS [J].

BORTHWICK, AC ;

HOLMS, WH ;

NIMMO, HG .

BIOCHEMICAL JOURNAL, 1984, 222 (03) :797-804

[2] GENETIC CONTROL OF ISOCITRATE LYASE ACTIVITY IN ESCHERICHIA COLI [J].

BRICE, CB ;

KORNBERG, HL .

JOURNAL OF BACTERIOLOGY, 1968, 96 (06) :2185-+

[3] GLYOXYLATE BYPASS OPERON OF ESCHERICHIA-COLI - CLONING AND DETERMINATION OF THE FUNCTIONAL MAP [J].

CHUNG, T ;

KLUMPP, DJ ;

LAPORTE, DC .

JOURNAL OF BACTERIOLOGY, 1988, 170 (01) :386-392

[4] REGULATION OF THE ACETATE OPERON IN ESCHERICHIA-COLI - PURIFICATION AND FUNCTIONAL-CHARACTERIZATION OF THE ICIR REPRESSOR [J].

CORTAY, JC ;

NEGRE, D ;

GALINIER, A ;

DUCLOS, B ;

PERRIERE, G ;

COZZONE, AJ .

EMBO JOURNAL, 1991, 10 (03) :675-679

[5] PHOSPHORYLATION OF ISOCITRATE DEHYDROGENASE OF ESCHERICHIA-COLI [J].

GARNAK, M ;

REEVES, HC .

SCIENCE, 1979, 203 (4385) :1111-1112

[6] Autoregulation of iclR, the gene encoding the repressor of the glyoxylate bypass operon [J].

Gui, LZ ;

Sunnarborg, A ;

Pan, B ;

LaPorte, DC .

JOURNAL OF BACTERIOLOGY, 1996, 178 (01) :321-324

[7] COMPILATION AND ANALYSIS OF ESCHERICHIA-COLI PROMOTER DNA-SEQUENCES [J].

HAWLEY, DK ;

MCCLURE, WR .

NUCLEIC ACIDS RESEARCH, 1983, 11 (08) :2237-2255

[8]

Higuchi R., 1990, PCR PROTOCOLS GUIDE

[9]

KORNBERG HL, 1967, B SOC CHIM BIOL, V49, P1479

[10]

KRAFT R, 1988, BIOTECHNIQUES, V6, P544