Soluble apyrases release ADP during ATP hydrolysis

被引：54

作者：

Chen, W ^{[1
]}

Guidotti, G ^{[1
]}

机构：

[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 282卷 / 01期

关键词：

CD39; E-NTPDase; apyrase; ADP; release; binding site; mechanism;

D O I：

10.1006/bbrc.2001.4555

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A soluble form of CD39 was expressed and purified from High-Five insect cells. The soluble CD39 is a monomer with a molecular weight of 54,000. The k(cat) and K-m of the purified soluble CD39 were 4.6 s(-1) and 12 muM for ATP and 1.3 s(-1) and 7 muM for ADP as substrates, respectively. One nucleotide binding site was detected on the monomer only in the presence of Ca2+. In contrast to the membrane bound CD39, soluble CD39 released ADP as an intermediate during ATP hydrolysis, as did the soluble potato apyrase, (C) 2001 Academic Press.

引用

页码：90 / 95

页数：6

共 21 条

[1] Ames B., 1966, METHOD ENZYMOL, V8, P115, DOI DOI 10.1016/0076-6879(66)08014-5
[2] AN ATPASE DOMAIN COMMON TO PROKARYOTIC CELL-CYCLE PROTEINS, SUGAR KINASES, ACTIN, AND HSP70 HEAT-SHOCK PROTEINS
BORK, P
SANDER, C
VALENCIA, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) : 7290 - 7294
[3] Intracellular localization of Na,K-ATPase alpha 2 subunit mutants
Coppi, MV
Guidotti, G
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1997, 346 (02) : 312 - 321
[4] Structural elements and limited proteolysis of CD39 influence ATP diphosphohydrolase activity
Esch, JSA
Sévigny, J
Kaczmarek, E
Siegel, JB
Imai, M
Koziak, K
Beaudoin, AR
Robson, SC
[J]. BIOCHEMISTRY, 1999, 38 (08) : 2248 - 2258
[5] Inhibition of platelet function by recombinant soluble ecto-ADPase/CD39
Gayle, RB
Maliszewski, CR
Gimpel, SD
Schoenborn, MA
Caspary, RG
Richards, C
Brasel, K
Price, V
Drosopoulos, JHF
Islam, N
Alyonycheva, TN
Broekman, MJ
Marcus, AJ
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (09) : 1851 - 1859
[6] Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum)
Handa, M
Guidotti, G
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 218 (03) : 916 - 923
[7] Hartwick R A, 1980, Adv Clin Chem, V21, P25, DOI 10.1016/S0065-2423(08)60086-4
[8] Functional characterization of rat ecto-ATPase and ecto-ATP diphosphohydrolase after heterologous expression in CHO cells
Heine, P
Braun, N
Heilbronn, A
Zimmermann, H
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 262 (01): : 102 - 107
[9] Identification and characterization of CD39 vascular ATP diphosphohydrolase
Kaczmarek, E
Koziak, K
Sevigny, J
Siegel, JB
Anrather, J
Beaudoin, AR
Bach, FH
Robson, SC
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (51) : 33116 - 33122
[10] A PHENOTYPIC STUDY OF CELLS FROM BURKITT-LYMPHOMA AND EBV-B-LYMPHOBLASTOID LINES AND THEIR RELATIONSHIP TO CELLS IN NORMAL LYMPHOID-TISSUES
LING, NR
HARDIE, D
LOWE, J
JOHNSON, GD
KHAN, M
MACLENNAN, ICM
[J]. INTERNATIONAL JOURNAL OF CANCER, 1989, 43 (01) : 112 - 118

← 1 2 3 →