Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis

被引：245

作者：

Rabilloud, T ^{[1
]}

机构：

[1] CEA Grenoble, DBMS, BECP, Lab Bioenerget Cellulaire & Pathol,UA 2019, F-38054 Grenoble 9, France

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 05期

关键词：

membrane solubilization; thiourea; two-dimensional polyacrylamide gel electrophoresis;

D O I：

10.1002/elps.1150190526

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of membrane proteins by high-resolution two-dimensional electrophoresis was carried out. At high loads, these proteins are prone to precipitation, resulting in poor resolution. It is shown here that the use of thiourea, previously described for focusing in immobilized pH gradients, can be extended to conventional isoelectric focusing. As thiourea inhibits acrylamide polymerization, a modified photopolymerization system must be used. These modifications result in higher solubility of proteins during IEF, thereby increasing the resolution and capacity of the two-dimensional gels.

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收藏

页码：758 / 760

页数：3

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