Egg contamination by Salmonella serovar enteritidis following vaccination with Δ-aroA Salmonella serovar typhimurium

被引:17
作者
Parker, C
Asokan, K
Guard-Petter, J
机构
[1] ARS, USDA, SEPRL, Athens, GA 30605 USA
[2] Univ Georgia, Dept Med Microbiol, Athens, GA 30602 USA
关键词
Salmonella; chicken; egg; immunization;
D O I
10.1111/j.1574-6968.2001.tb10500.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The efficacy of an aroA Solmonella serovar tyhphimurium modified live vaccine to decrease internal egg contamination after oral challenge of hens with egg-contaminating Salmonella serovar enteritidis was assessed. Challenge was with a mixed phenotype of S. enteritidis that had virulence characteristics previously associated with enhanced oral invasiveness and egg contamination in chickens. Immunized birds had fewer positive ovary/oviduct pools and lower cfu g(-1) cecal contents than did non-immunized birds, but the differences were not significant. The number of positive intestinal (duodenum, jejunum, ileum) and organ (spleen, kidney, liver) pools following challenge from each treatment group were equivalent. Most importantly. immunization did not decrease egg contamination. These results suggest that the ability of modified live vaccines to reduce internal egg contamination by S. serovar enteritidis can be assessed using characterized strains for challenge. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:73 / 78
页数:6
相关论文
共 22 条
[1]   SALMONELLA REFERENCE COLLECTION-B (SARB) - STRAINS OF 37 SEROVARS OF SUBSPECIES-I [J].
BOYD, EF ;
WANG, FS ;
BELTRAN, P ;
PLOCK, SA ;
NELSON, K ;
SELANDER, RK .
JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 :1125-1132
[2]   VACCINATION OF CHICKENS WITH STRAIN CVL30, A GENETICALLY DEFINED SALMONELLA-ENTERITIDIS AROA LIVE ORAL VACCINE CANDIDATE [J].
COOPER, GL ;
VENABLES, LM ;
WOODWARD, MJ ;
HORMAECHE, CE .
INFECTION AND IMMUNITY, 1994, 62 (11) :4747-4754
[3]   LIVE ORAL AVIRULENT SALMONELLA VACCINES [J].
CURTISS, R ;
KELLY, SM ;
HASSAN, JO .
VETERINARY MICROBIOLOGY, 1993, 37 (3-4) :397-405
[4]   Intestinal colonization and organ invasion in chicks experimentally infected with Salmonella enteritidis phage type 4 and other phage types isolated from poultry in the United States [J].
Gast, RK ;
Benson, ST .
AVIAN DISEASES, 1996, 40 (04) :853-857
[5]  
Guard-Petter J, 1998, APPL ENVIRON MICROB, V64, P2166
[6]   A novel relationship between O-antigen variation, matrix formation, and invasiveness of Salmonella enteritidis [J].
GuardPetter, J ;
Keller, LH ;
Rahman, MM ;
Carlson, RW ;
Silvers, S .
EPIDEMIOLOGY AND INFECTION, 1996, 117 (02) :219-231
[7]   Efficacy of a live avirulent Salmonella typhimurium vaccine in preventing colonization and invasion of laying hens by Salmonella typhimurium and Salmonella enteritidis [J].
Hassan, JO ;
Curtiss, R .
AVIAN DISEASES, 1997, 41 (04) :783-791
[8]  
Henzler DJ, 1998, AM J VET RES, V59, P824
[9]   Epidemiology and control of egg-associated Salmonella Enteritidis in the United States of America [J].
Hogue, A ;
White, P ;
Guard-Petter, J ;
Schlosser, W ;
Gast, R ;
Ebel, E ;
Farrar, J ;
Gomez, T ;
Madden, J ;
Madison, M ;
McNamara, AM ;
Morales, R ;
Parham, D ;
Sparling, P ;
Sutherlin, W ;
Swerdlow, D .
REVUE SCIENTIFIQUE ET TECHNIQUE DE L OFFICE INTERNATIONAL DES EPIZOOTIES, 1997, 16 (02) :542-553
[10]   Protection against oral challenge three months after iv immunization of BALB/c mice with live Aro Salmonella typhimurium and Salmonella enteritidis vaccines is serotype (species)-dependent and only partially determined by the main LPS O antigen [J].
Hormaeche, CE ;
Mastroeni, P ;
Harrison, JA ;
deHormaeche, RD ;
Svenson, S ;
Stocker, BAD .
VACCINE, 1996, 14 (04) :251-259