Adding New Chemistries to the Genetic Code

被引：1317

作者：

Liu, Chang C. ^{[1
]}

Schultz, Peter G. ^{[1
]}

机构：

[1] Scripps Res Inst, La Jolla, CA 92037 USA

来源：

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 79 | 2010年 / 79卷

关键词：

aminoacyl-tRNA synthetase; protein engineering; protein evolution; translation; TRANSFER-RNA SYNTHETASE; UNNATURAL AMINO-ACIDS; SITE-SPECIFIC INCORPORATION; IN-VIVO INCORPORATION; NEWLY SYNTHESIZED PROTEINS; SUPPRESSOR TRANSFER-RNA; PHOTO-CROSS-LINKING; LEUCYL-TRANSFER-RNA; ESCHERICHIA-COLI; EFFICIENT INCORPORATION;

D O I：

10.1146/annurev.biochem.052308.105824

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of new orthogonal aminoacyl-tRNA synthetase/tRNA pairs has led to the addition of approximately 70 unnatural amino acids (UAAs) to the genetic codes of Escherichia coli, yeast, and mammalian cells. These UAAs represent a wide range of structures and functions not found in the canonical 20 amino acids and thus provide new opportunities to generate proteins with enhanced or novel properties and probes of protein structure and function.

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页码：413 / 444

页数：32

相关论文

共 187 条

[1] Site-specific incorporation of a redox-active amino acid into proteins [J].

Alfonta, L ;

Zhang, ZW ;

Uryu, S ;

Loo, JA ;

Schultz, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) :14662-14663

[2] Natural expansion of the genetic code [J].

Ambrogelly, Alexandre ;

Palioura, Sotiria ;

Soll, Dieter .

NATURE CHEMICAL BIOLOGY, 2007, 3 (01) :29-35

[3] An expanded genetic code with a functional quadruplet codon [J].

Anderson, JC ;

Wu, N ;

Santoro, SW ;

Lakshman, V ;

King, DS ;

Schultz, PG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (20) :7566-7571

[4] Adaptation of an orthogonal archaeal leucyl-tRNA and synthetase pair for four-base, amber, and opal suppression [J].

Anderson, JC ;

Schultz, PG .

BIOCHEMISTRY, 2003, 42 (32) :9598-9608

[5]

[Anonymous], 2005, POSTTRANSLATIONAL MO

[6] CATALYTIC CONTRIBUTION OF FLAP-SUBSTRATE HYDROGEN-BONDS IN HIV-1 PROTEASE EXPLORED BY CHEMICAL SYNTHESIS [J].

BACA, M ;

KENT, SBH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) :11638-11642

[7] Expansion of the genetic code enables design of a novel "gold'' class of green fluorescent proteins [J].

Bae, JH ;

Rubini, M ;

Jung, G ;

Wiegand, G ;

Seifert, MHJ ;

Azim, MK ;

Kim, JS ;

Zumbusch, A ;

Holak, TA ;

Moroder, L ;

Huber, R ;

Budisa, N .

JOURNAL OF MOLECULAR BIOLOGY, 2003, 328 (05) :1071-1081

[8] RIBOSOME-MEDIATED INCORPORATION OF A NONSTANDARD AMINO-ACID INTO A PEPTIDE THROUGH EXPANSION OF THE GENETIC-CODE [J].

BAIN, JD ;

SWITZER, C ;

CHAMBERLIN, AR ;

BENNER, SA .

NATURE, 1992, 356 (6369) :537-539

[9] Selective dye-labeling of newly synthesized proteins in bacterial cells [J].

Beatty, KE ;

Xie, F ;

Wang, Q ;

Tirrell, DA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (41) :14150-14151

[10] Fluorescence visualization of newly synthesized proteins in mammalian cells [J].

Beatty, Kimberly E. ;

Liu, Julie C. ;

Xie, Fang ;

Dieterich, Daniela C. ;

Schuman, Erin M. ;

Wang, Qian ;

Tirrell, David A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (44) :7364-7367

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