Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity

被引：229

作者：

Huang, Sheng-quan ^{[2
]}

Ning, Zheng-xiang ^{[1
]}

机构：

[1] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong Prov, Peoples R China

[2] Infinitus China Co Ltd, Jiangmen 529156, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2010年 / 47卷 / 03期

关键词：

Polysaccharide; Extraction; Immunoassay; RESPONSE-SURFACE METHODOLOGY; ASSISTED EXTRACTION; ANTITUMOR-ACTIVITY; OPTIMIZATION; PURIFICATION; LYMPHOCYTES; MUSHROOM;

D O I：

10.1016/j.ijbiomac.2010.03.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In the present study, in order to maximize the yield of polysaccharides from Ganoderma lucidum, response surface methodology was employed to optimize the ultrasonic/microwave-assisted extraction (UMAE) conditions. The results indicated that the optimal extraction conditions were ultrasonic power of 50 W, microwave power of 284 W, extraction time of 701 s and water/solid ratio of 11.6:1, respectively. Using UMAE, the yield of polysaccharides was 115.56% above that of classical hot water extraction (HWE) and increased by 27.7% as against ultrasound-assisted extraction (UAE), which confirmed the great potential application of UMAE technology in the extraction of polysaccharides. The immunological assays results demonstrated that polysaccharides of G. lucidum extracted by ultrasonic/microwave (UMP) could improve the weight of immune organ of immunocompromised mice, restore delayed-type hypersensitivity (DTH) reaction to DFNB, improve hemolysis antibody level and natural killer cell activity at high-dose. However, UMP had no noticeable effects on phagocytosis of monocyte at the tested dosage range. (C) 2010 Published by Elsevier B.V.

引用

页码：336 / 341

页数：6

共 28 条

[1]

Purification, characterization, and modification of T lymphocyte-stimulating polysaccharide from spores of Ganoderma lucidum [J].

Bao, XF ;

Zhen, Y ;

Ruan, L ;

Fang, JN .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2002, 50 (05) :623-629

[2]

Structural features of immunologically active polysaccharides from Ganoderma lucidum [J].

Bao, XF ;

Wang, XS ;

Dong, Q ;

Fang, JN ;

Li, XY .

PHYTOCHEMISTRY, 2002, 59 (02) :175-181

[3]

Chemical reactions under "non-classical conditions", microwaves and ultrasound in the synthesis of vitamins [J].

Bonrath, W .

ULTRASONICS SONOCHEMISTRY, 2004, 11 (01) :1-4

[4]

Extraction, purification, and characterization of the polysaccharides from Opuntia milpa alta [J].

Cai, Weirong ;

Gu, Xiaohong ;

Tang, Jian .

CARBOHYDRATE POLYMERS, 2008, 71 (03) :403-410

[5]

Microwave-assisted hydrolysis of Zymomonas mobilis levan envisaging oligofructan production [J].

de Paula, Valdemir Cordeiro ;

Pinheiro, Irapuan Oliveira ;

Lopes, Carlos Edison ;

Torres Calazans, Glicia Maria .

BIORESOURCE TECHNOLOGY, 2008, 99 (07) :2466-2470

[6]

Application of Box-Behnken design in optimisation for polysaccharides extraction from cultured mycelium of Cordyceps sinensis [J].

Dong, C. -H. ;

Xie, X. -Q ;

Wang, X. -L. ;

Zhan, Y. ;

Yao, Y-J. .

FOOD AND BIOPRODUCTS PROCESSING, 2009, 87 (C2) :139-144

[7]

A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[8]

POLYSACCHARIDES IN PHARMACY - CURRENT APPLICATIONS AND FUTURE CONCEPTS [J].

FRANZ, G .

PLANTA MEDICA, 1989, (06) :493-497

[9]

ANTITUMOR-ACTIVITY OF GANODERMA-LUCIDUM, AN EDIBLE MUSHROOM, ON INTRAPERITONEALLY IMPLANTED LEWIS LUNG-CARCINOMA IN SYNERGENIC MICE [J].

FURUSAWA, E ;

CHOU, SC ;

FURUSAWA, S ;

HIRAZUMI, A ;

DANG, Y .

PHYTOTHERAPY RESEARCH, 1992, 6 (06) :300-304

[10]

Optimisation and characterisation of bioadhesive controlled release tetracycline microspheres [J].

Govender, S ;

Pillay, V ;

Chetty, DJ ;

Essack, SY ;

Dangor, CM ;

Govender, T .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2005, 306 (1-2) :24-40

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