A putative HCO-3 transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

被引：54

作者：

Bonfil, DJ

Ronen-Tarazi, M

Sültemeyer, D

Lieman-Hurwitz, J

Schatz, D

Kaplan, A ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Moshe Shilo Ctr, Dept Plant Sci, IL-91904 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Avron Evenari MInerva Ctr, IL-91904 Jerusalem, Israel

[3] Universitat Kaiserslautern, Fachbereich Biol, D-67653 Kaiserslautern, Germany

来源：

FEBS LETTERS | 1998年 / 430卷 / 03期

基金：

以色列科学基金会;

关键词：

CO2-concentrating mechanism; crossover; cyanobacteria; HCO3-; uptake; ictB;

D O I：

10.1016/S0014-5793(98)00662-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells, An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2, IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3- uptake in the wild type and IL-2 suggested the presence of two or three HCO3- carriers exhibiting different affinities to HCO3-. (C) 1998 Federation of European Biochemical Societies.

引用

页码：236 / 240

页数：5

共 29 条

[1] THE CO2 CONCENTRATING MECHANISM IN CYANOBACTERIA AND MICROALGAE
BADGER, MR
PRICE, GD
[J]. PHYSIOLOGIA PLANTARUM, 1992, 84 (04) : 606 - 615
[2] THE ROLE OF CARBONIC-ANHYDRASE IN PHOTOSYNTHESIS
BADGER, MR
PRICE, GD
[J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1994, 45 : 369 - 392
[3] INSERTIONAL INACTIVATION OF GENES TO ISOLATE MUTANTS OF SYNECHOCOCCUS SP STRAIN PCC-7942 - ISOLATION OF FILAMENTOUS STRAINS
DOLGANOV, N
GROSSMAN, AR
[J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (23) : 7644 - 7651
[4] A GENE HOMOLOGOUS TO CHLOROPLAST CARBONIC-ANHYDRASE (ICFA) IS ESSENTIAL TO PHOTOSYNTHETIC CARBON-DIOXIDE FIXATION BY SYNECHOCOCCUS PCC7942
FUKUZAWA, H
SUZUKI, E
KOMUKAI, Y
MIYACHI, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) : 4437 - 4441
[5] Kaneko T, 1996, DNA Res, V3, P109
[6] PHYSIOLOGICAL AND MOLECULAR ASPECTS OF THE INORGANIC CARBON-CONCENTRATING MECHANISM IN CYANOBACTERIA
KAPLAN, A
SCHWARZ, R
LIEMANHURWITZ, J
REINHOLD, L
[J]. PLANT PHYSIOLOGY, 1991, 97 (03) : 851 - 855
[7] Kaplan A., 1994, MOL BIOL CYANOBACTER, P469, DOI 10.1007/978-94-011-0227-8_15
[8] KAPLAN A, 1988, METHOD ENZYMOL, P534
[9] KAPLAN A, 1998, IN PRESS CAN J BOT
[10] KAPLAN A, 1990, BOT MAG TOKYO, V2, P53

← 1 2 3 →