Mining metagenomes for novel cellulase genes

被引:75
作者
Duan, Cheng-Jie [1 ,2 ,3 ]
Feng, Jia-Xun [1 ,2 ,3 ]
机构
[1] Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, Key Lab Minist Educ Microbial & Plant Genet Engn, Nanning 530004, Guangxi, Peoples R China
[2] Guangxi Univ, Coll Life Sci & Technol, Nanning 530004, Guangxi, Peoples R China
[3] Guangxi Acad Sci, Natl Engn Res Ctr Nonfood Biorefinery, Cellulose Proc Lab, Nanning 530003, Guangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Biotechnological applications; Cellulase; Function-based approach; Metagenomics; Sequencing; BETA-GLUCOSIDASE; RETICULITERMES-SPERATUS; ENVIRONMENTAL DNA; SOIL METAGENOME; RABBIT CECUM; IDENTIFICATION; DEGRADATION; RUMEN; ENZYMES; LIGNOCELLULOSE;
D O I
10.1007/s10529-010-0356-z
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cellulases hydrolyze the beta-1,4 linkages of cellulose and are widely used in food, brewing and wine, animal feed, textiles and laundry, and pulp and paper industries, especially for hydrolyzing cellulosic materials into sugars, which can be fermented to produce useful products such as ethanol. Metagenomics has become an alternative approach to conventional culture-dependent methods as it allows exhaustive mining of microbial genomes in their natural environments. This review covers the current state of research and challenges in mining novel cellulase genes from the metagenomes of various environments, and discusses the potential biotechnological applications of metagenome-derived cellulases.
引用
收藏
页码:1765 / 1775
页数:11
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