Cylindrical compact thermal-cycling device for continuous-flow polymerase chain reaction

被引：89

作者：

Park, N ^{[1
]}

Kim, S ^{[1
]}

Hahn, JH ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Chem, Div Mol & Life Sci, Natl Res Lab Adv Biotechnol & Biomed Microinstrum, Pohang 790784, South Korea

来源：

ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 21期

关键词：

D O I：

10.1021/ac0346959

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A compact, thermal-cycling device for high-throughput continuous-flow polymerase chain reaction (PCR) has been developed, which consists of a flow-through capillary and a cylindrical heating-block assembly. A 3.5-m-long fused-silica capillary coils helically, with 33 turns, up around the 30-mm-diameter assembly of three equally divided thermostating copper blocks for melting, annealing, and extension. An injected PCR mixture undergoes one cycle of PCR each turn. A continuous-flow PCR of one sample and also a segmented-flow PCR of four different samples have been successfully demonstrated. The present device can easily evolve into a parallel-processing, multistation compact device and be modified to have real-time PCR capability. Ibis solid-based compact PCR device, therefore, has a potentiality to be the format of choice when developed for a portable system.

引用

页码：6029 / 6033

页数：5

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