Immobilization of β-Glucosidase on carbon nanotubes

被引：56

作者：

Gómez, JM ^{[1
]}

Romero, MD ^{[1
]}

Fernández, TM ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Chem, Dept Chem Engn, E-28040 Madrid, Spain

来源：

CATALYSIS LETTERS | 2005年 / 101卷 / 3-4期

关键词：

beta-Glucosidase; carbon nanotubes; immobilization; activity;

D O I：

10.1007/s10562-005-4904-4

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Carbon nanotubes are demonstrated as a good support for the immobilization of β-Glucosidase. This is an enzyme with high molecular weight (ca. 135 kDa). A high enzyme loading of 630 mg per gram of support was achieved in 12 h. The link between the enzyme and the carbon nanotubes surface was by electrostatic interactions due to the different charges of the enzyme and the support at the pH of the immobilization. Immobilized β-Glucosidase showed a catalytic activity above 400 U/g on the hydrolysis of 4-nitrophenyl-β-D-glucopyranoside (p-NPG).

引用

页码：275 / 278

页数：4

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