Escherichia coli RNA polymerase cove and holoenzyme structures

被引:35
作者
Finn, RD
Orlova, EV
Gowen, B
Buck, M
van Heel, M [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AY, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol, London SW7 2AY, England
关键词
core RNA polymerase; cryo-electron microscopy; sigma(70) holoenzyme;
D O I
10.1093/emboj/19.24.6833
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Multisubunit RNA polymerase is an essential enzyme for regulated gene expression. Here we report two Escherichia coli RNA polymerase structures: an 11.0 Angstrom structure of the core RNA polymerase and a 9.5 Angstrom structure of the sigma (70) holoenzyme. Both structures were obtained by cryo-electron microscopy and angular reconstitution. Core RNA polymerase exists in an open conformation. Extensive conformational changes occur between the core and the holoenzyme forms of the RNA polymerase, which are largely associated with movements in beta'. An common RNA polymerase subunits (alpha (2), beta, beta') could be localized in both structures, thus suggesting the position of sigma (70) in the holoenzyme.
引用
收藏
页码:6833 / 6844
页数:12
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