Optimisation of wort volatile analysis by headspace solid-phase microextraction in combination with gas chromatography and mass spectrometry

被引：33

作者：

De Schutter, David P. ^{[1
]}

Saison, Daan ^{[1
]}

Delvaux, Filip ^{[1
]}

Derdelinckx, Guy ^{[1
]}

Rock, Jean-Marie ^{[2
]}

Neven, Hedwig ^{[3
]}

Delvaux, Freddy R. ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Biosci Engn, Ctr Malting & Brewing Sci, B-3001 Heverlee, Belgium

[2] Brasserie Orval SA, B-6823 Villers Devant Orval, Belgium

[3] Brewery Duvel Moortgat NV, B-2870 Puurs, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1179卷 / 02期

关键词：

wort; beer; solid-phase microextraction; volatiles; aldehydes; wort boiling; gas chromatography; mass spectrometry;

D O I：

10.1016/j.chroma.2007.11.103

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to create a simple, solventless technique without derivatisation in order to analyze a broad range of volatiles in beer wort. A method was developed using headspace solid-phase microextraction coupled with gas chromatography and mass spectrometry. The procedure was optimised by selection of the appropriate fibre and optimisation of extraction temperature, extraction time, and salting-out. The detection limits were well below the actual wort concentrations of the selected volatiles, ranging from 12 ng/l for linalool to 0.53 mu g/l for furfural. Moreover, the procedure showed a good linearity and was applied to the analysis of wort samples taken from a wort boiling process in an industrial brewery. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：75 / 80

页数：6

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