Persistence of Environmental DNA in Freshwater Ecosystems

被引:434
作者
Dejean, Tony [1 ,2 ,3 ]
Valentini, Alice [1 ,3 ]
Duparc, Antoine [3 ]
Pellier-Cuit, Stephanie [4 ]
Pompanon, Francois [4 ]
Taberlet, Pierre [4 ]
Miaud, Claude [3 ]
机构
[1] SPYGEN, Savoie Technolac, Le Bourget Du Lac, France
[2] Parc Nat Reg Perigord Limousin, La Coquille, France
[3] Univ Savoie, UMR CNRS 5553, Lab Ecol Alpine, Le Bourget Du Lac, France
[4] Univ Grenoble 1, UMR CNRS 5553, Lab Ecol Alpine, Grenoble, France
来源
PLOS ONE | 2011年 / 6卷 / 08期
关键词
PLASMID DNA; PLANT DNA; ANCIENT; TRANSFORMATION; INFERENCES; STABILITY;
D O I
10.1371/journal.pone.0023398
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The precise knowledge of species distribution is a key step in conservation biology. However, species detection can be extremely difficult in many environments, specific life stages and in populations at very low density. The aim of this study was to improve the knowledge on DNA persistence in water in order to confirm the presence of the focus species in freshwater ecosystems. Aquatic vertebrates (fish: Siberian sturgeon and amphibian: Bullfrog tadpoles) were used as target species. In control conditions (tanks) and in the field (ponds), the DNA detectability decreases with time after the removal of the species source of DNA. DNA was detectable for less than one month in both conditions. The density of individuals also influences the dynamics of DNA detectability in water samples. The dynamics of detectability reflects the persistence of DNA fragments in freshwater ecosystems. The short time persistence of detectable amounts of DNA opens perspectives in conservation biology, by allowing access to the presence or absence of species e. g. rare, secretive, potentially invasive, or at low density. This knowledge of DNA persistence will greatly influence planning of biodiversity inventories and biosecurity surveys.
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页数:4
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