Protocol for effective differentiation of 3T3-L1 cells to adipocytes

被引：522

作者：

Zebisch, Katja ^{[1
]}

Voigt, Valerie ^{[1
]}

Wabitsch, Martin ^{[2
]}

Brandsch, Matthias ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Biozentrum, D-06120 Halle, Saale, Germany

[2] Univ Ulm, Dept Pediat & Adolescent Med, Div Pediat Endocrinol & Diabet, D-89075 Ulm, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 425卷 / 01期

关键词：

3T3-L1; cells; Adipocyte differentiation; Rosiglitazone; ADIPOSE CONVERSION; PREADIPOCYTE; CULTURE; LINE;

D O I：

10.1016/j.ab.2012.03.005

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

In this note, we present a detailed procedure for highly effective and reproducible 313-L1 cell differentiation. Due to their potential to differentiate from fibroblasts to adipocytes, 313-L1 cells are widely used for studying adipogenesis and the biochemistry of adipocytes. However, using different kits and protocols published so far, we were not able to obtain full differentiation of the currently available American Type Culture Collection (ATCC) 313-L1 cell lots. Using rosiglitazone (2 mu M) as an additional prodifferentiative agent, we achieved apparently complete differentiation of 313-L1 cells within 10 to 12 days that persisted for at least up to cell culture passage 10. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：88 / 90

页数：3

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