An evaluation of 3C-based methods to capture DNA interactions

被引：221

作者：

Simonis, Marieke ^{[1
]}

Kooren, Jurgen ^{[1
]}

de Laat, Wouter ^{[1
]}

机构：

[1] Erasmus MC, Dept Cell Biol & Genet, NL-3000 CA Rotterdam, Netherlands

来源：

NATURE METHODS | 2007年 / 4卷 / 11期

关键词：

D O I：

10.1038/NMETH1114

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The shape of the genome is thought to play an important part in the coordination of transcription and other DNA-metabolic processes. Chromosome conformation capture (3C) technology allows us to analyze the folding of chromatin in the native cellular state at a resolution beyond that provided by current microscopy techniques. It has been used, for example, to demonstrate that regulatory DNA elements communicate with distant target genes through direct physical interactions that loop out the intervening chromatin fiber. Here we discuss the intricacies of 3C and new 3C-based methods including the 4C, 5C and ChIP-loop assay.

引用

页码：895 / 901

页数：7

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