Characterization of the O antigen gene cluster and structural analysis of the O antigen of Francisella tularensis subsp tularensis

被引:64
作者
Prior, JL [1 ]
Prior, RG
Hitchen, PG
Diaper, H
Griffin, KF
Morris, HR
Dell, A
Titball, RW
机构
[1] Dstl Porton Down, Salisbury SP4 0JQ, Wilts, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England
[3] M SCAN Mass Spect Res & Training Ctr, Ascot SL5 7PZ, Berks, England
[4] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England
关键词
D O I
10.1099/jmm.0.05184-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A gene cluster encoding enzymes involved in LPS O antigen biosynthesis was identified from the partial genome sequence of Francisella tularensis subsp. tularensis Schu S4. All of the genes within the cluster were assigned putative functions based on sequence similarity with genes from O antigen biosynthetic clusters from other bacteria. Ten pairs of overlapping primers were designed to amplify the O antigen biosynthetic cluster by PCR from nine strains of F. tularensis. Although the gene cluster was present in all strains, there was a size difference in one of the PCR products between subsp. tularensis strains and subsp. holarctica strains. LPS was purified from F. tularensis subsp, tularensis Schu S4 and the O antigen was shown by mass spectrometry to have a structure similar to that of F. tularensis subsp. holarctica strain 15. When LPS from F. tularensis subsp. tularensis Schu S4 was used to immunize mice that were then challenged with F. tularensis subsp. tularensis Schu S4, an extended time to death was observed.
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收藏
页码:845 / 851
页数:7
相关论文
共 33 条
[1]   Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine [J].
Albermann, C ;
Piepersberg, W .
GLYCOBIOLOGY, 2001, 11 (08) :655-661
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[4]   Inability of the Francisella tularensis lipopolysaccharide to mimic or to antagonize the induction of cell activation by endotoxins [J].
Ancuta, P ;
Pedron, T ;
Girard, R ;
Sandstrom, G ;
Chaby, R .
INFECTION AND IMMUNITY, 1996, 64 (06) :2041-2046
[5]   Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide [J].
Bélanger, M ;
Burrows, LL ;
Lam, JS .
MICROBIOLOGY-UK, 1999, 145 :3505-3521
[6]  
CHART H, 1994, METHODS PRACTICAL LA, P11
[7]   Phase variation in Francisella tularensis affecting intracellular growth, lipopolysaccharide antigenicity and nitric oxide production [J].
Cowley, SC ;
Myltseva, SV ;
Nano, FE .
MOLECULAR MICROBIOLOGY, 1996, 20 (04) :867-874
[8]  
DELL A, 1994, METHOD ENZYMOL, V230, P108
[9]   ANALYSIS OF OLIGOSACCHARIDE EPITOPES OF MENINGOCOCCAL LIPOPOLYSACCHARIDES BY FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY [J].
DELL, A ;
AZADI, P ;
TILLER, P ;
THOMASOATES, J ;
JENNINGS, HJ ;
BEURRET, M ;
MICHON, F .
CARBOHYDRATE RESEARCH, 1990, 200 :59-76
[10]   Purified lipopolysaccharide from Francisella tularensis live vaccine strain (LVS) induces protective immunity against LVS infection that requires B cells and gamma interferon [J].
Dreisbach, VC ;
Cowley, S ;
Elkins, KL .
INFECTION AND IMMUNITY, 2000, 68 (04) :1988-1996