The Rhizobium etli argC gene is essential for arginine biosynthesis and nodulation of Phaseolus vulgaris

被引：12

作者：

Ferraioli, S ^{[1
]}

Taté, R ^{[1
]}

Caputo, E ^{[1
]}

Lamberti, A ^{[1
]}

Riccio, A ^{[1
]}

Patriarca, EJ ^{[1
]}

机构：

[1] CNR, Int Inst Genet & Biophys, I-80125 Naples, Italy

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2001年 / 14卷 / 02期

关键词：

polyamines biosynthesis; root exudates;

D O I：

10.1094/MPMI.2001.14.2.250

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Tn5-induced mutant strain (CTNUX5) of Rhizobium etli unable to grow with ammonium as the sole nitrogen source was isolated and characterized, Sequence analysis showed that Tn5 is inserted into an argC-homologous gene. Unlike its wild-type parent (strain CE3), the mutant strain CTNUX5 had an absolute dependency on arginine to grow. The argC gene was cloned from the wild-type strain CE3, and the resulting plasmid, pAR207, after transformation was shown to relieve the arginine auxotrophy of strain CTNUX5. Unlike strain CE3 or CTNUX5-pAR207, strain CTNUX5 showed undetectable levels of N-acetyl-gamma -glutamylphosphate reductase activity. Unless arginine was added to the growth medium, strain CTNUX5 was unable to produce flavonoid-inducible lipo-chitin oligosaccharides (nodulation factors) and to induce nodules or nodulelike structures on the roots of Phaseolus vulgaris.

引用

页码：250 / 254

页数：5

共 21 条

[1] Isolation, chemical structures and biological activity of the lipo-chitin oligosaccharide nodulation signals from Rhizobium etli [J].