Purification of apolipoprotein H(beta2-glycoprotein I)-like protein from human follicular fluid

被引：9

作者：

Aleporou-Marinou, V ^{[1
]}

Pappa, H ^{[1
]}

Yalouris, P ^{[1
]}

Patargias, T ^{[1
]}

机构：

[1] Univ Athens, Dept Biol, Div Gen & Biotechnol, GR-15701 Athens, Greece

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2001年 / 128卷 / 03期

关键词：

apolipoprotein H; beta 2-glycoprotein I; fertilization process; follicular fluid; in vitro fertilization; sperm motility; computer assisted semen analysis; HPLC chromatography;

D O I：

10.1016/S1096-4959(00)00359-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We purified a glycoprotein of molecular weight 50 kDa that has an N-terminal sequence similar to that of apolipoprotein H indicating that it is identical to or highly homologous to apolipoprotein H. There are indications that apolipoprotein H or its homologue may be involved in the fertilization process. Sperm motion was assessed employing computer-assisted semen analysis. The addition of the purified protein to prepared sperm samples from normospermic men increases significantly the straight line velocity (VSL) and the amplitude of lateral head displacement (ALH) but does not increase the number of progressively motile sperm. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：537 / 542

页数：6

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