Potato germplasm: in vitro storage and virus reduction

An efficient protocol for in vitro longterm storage of a potato (Solanum tuberosum:) germplasm collection has been devised. Potato explants were prepared for tissue culture and then underwent chemical (using the antiviral chemical, ribavirin) and heat therapy. It combines previously reported protocols of in vitro virus elimination, micro tuber induction, and long-term storage and is especially suitable for conserving a large collection. Five weeks later, the apical tips were introduced to microtuber induction medium. After shoot and root development, the tissue was ELISA tested for confirmation of significant virus reduction and indexing, and then the plantlets were transferred to a cabinet set for microtuber induction. The microtubers formed after 12-16 weeks and then the potties were transferred into long-term storage conditions. A minimum storage time of 3 years has been achieved.