A simple laboratory experiment for teaching enzyme immobilization with urease and its application in blood urea estimation

被引：29

作者：

Kayastha, AM ^{[1
]}

Das, N ^{[1
]}

机构：

[1] Banaras Hindu Univ, Fac Sci, Sch Biotechnol, Varanasi 221005, Uttar Pradesh, India

来源：

BIOCHEMICAL EDUCATION | 1999年 / 27卷 / 02期

关键词：

D O I：

10.1016/S0307-4412(98)00231-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An experiment is described in which students carry out urease purification, immobilization and its application in blood urea estimation. Urease from pigeonpea is partially purified using acetone fractionation and then immobilized on calcium alginate in the form of beads. The immobilized enzyme has a better shelf-life at 4 degrees C than soluble enzyme. Various aspects of enzyme immobilization are discussed. Blood urea estimation is carried out with immobilized enzyme beads and the beads can be used repeatedly for this purpose making it an economical procedure compared to commercial kits. (C) 1999 IUBMB. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：114 / 117

页数：4

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