Order, dynamics and metal-binding in the lead-dependent ribozyme

被引：56

作者：

Legault, P ^{[1
]}

Hoogstraten, CG ^{[1
]}

Metlitzky, E ^{[1
]}

Pardi, A ^{[1
]}

机构：

[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1998年 / 284卷 / 02期

关键词：

ribozymes; catalytic RNA; leadzyme; RNA structure; chemical probing;

D O I：

10.1006/jmbi.1998.2181

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vitro selected lead-dependent ribozyme is among the smallest and simplest of the known catalytic RNA motifs and has a unique metal ion specificity for divalent lead. The conformation and dynamics of this ribozyme are analyzed here by NMR and chemical probing experiments. Complete assignments of the H-1, C-13 and N-15 resonances have been made, and the NMR chemical shift changes in the presence of Pb2+, Mg2+ or high concentrations of Na+ show that there is no significant structural change upon addition of either activating (Pb2+) or inhibitory (Mg2+) divalent ions. The C-13 NMR relaxation data indicate substantial dynamic fluctuations on various time-scales for active-site residues in this ribozyme. The combination of chemical probing and NMR experiments reveals a picture of the active site for the lead-dependent ribozyme that has both ordered and dynamic features. (C) 1998 Academic Press.

引用

页码：325 / 335

页数：11

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