The Haemophilus influenzae Hap serine protease promotes adherence and microcolony formation, potentiated by a soluble host protein

Haemophilus influenzae initiates infection by colonizing the upper respiratory mucosa. The process of colonization involves adherence to epithelium and evasion of host immunity. in this study, we examined the H. influenzae Map adhesin, which has serine protease activity and undergoes autoproteolytic cleavage and extracellular release in broth, We found that the uncleaved cell-associated form of Hap mediates adherence to cultured epithelial cells and promotes bacterial aggregation and microcolony formation. Adherence and aggregation are augmented by secretory leukocyte protease inhibitor, a natural component of respiratory secretions that inhibits nag autoproteolysis. These observations suggest a never paradigm in host-pathogen relations, in which a soluble host protein whose primary function is to protect host epithelium potentiates properties that facilitate bacterial colonization.