Lacticin 481: In vitro reconstitution of lantibiotic synthetase activity

被引：201

作者：

Xie, LL ^{[1
]}

Miller, LM ^{[1
]}

Chatterjee, C ^{[1
]}

Averin, O ^{[1
]}

Kelleher, NL ^{[1
]}

van der Donk, WA ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

SCIENCE | 2004年 / 303卷 / 5658期

关键词：

D O I：

10.1126/science.1092600

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The lantibiotic lacticin 481 is synthesized on ribosomes as a prepeptide (LctA) and posttranslationally modified to its mature form. These modi. cations include dehydration of serines and threonines, followed by intramolecular addition of cysteines to the unsaturated amino acids, which generates cyclic thioethers. This process breaks eight chemical bonds and forms six newbonds and is catalyzed by one enzyme, LctM. We have characterized the in vitro activity of LctM, which completely processed a series of LctA mutants, displaying a permissive substrate specificity that holds promise for antibiotic engineering.

引用

页码：679 / 681

页数：3

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