Molecular epidemiological study of a mass outbreak caused by enteropathogenic Escherichia coli O157:H45

被引：21

作者：

Makino, S

Asakura, H

Shirahata, T

Ikeda, T

Takeshi, K

Arai, K

Nagasawa, M

Abe, T

Sadamoto, T

机构：

[1] Obihiro Univ Agr & Vet Med, Dept Vet Microbiol, Obihiro, Hokkaido 0808555, Japan

[2] Hokkaido Inst Publ Hlth, Sapporo, Hokkaido 0600819, Japan

[3] Obihiro Publ Hlth Ctr, Obihiro, Hokkaido 0800803, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1999年 / 43卷 / 04期

关键词：

EPEC; HEp-2; adherence; O157;

D O I：

10.1111/j.1348-0421.1999.tb02419.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We made a molecular analysis of O157:H45 Escherichia coli isolated from a mass outbreak that occurred in Obihiro City, Using DNA analysis, we confirmed this infection case as a mass outbreak. Although the isolates expressed O157 antigen, they did not produce Vero toxin. We concluded they were enteropathogenic E. coli (EPEC) because they had a bfp gene and an EAF plasmid, and further they exhibited local adherence to HEp-2 cells. We believe this is the first report of a mass outbreak by O157 EPEC, and we suggest that PCR using eae- and bfp-specific primers and HEp-2 adherence assay are useful to identify EPEC.

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页码：381 / 384

页数：4

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