Pectin degradation in UF-membrane reactors with commercial pectinases

被引：7

作者：

Dinnella, C

Stagni, A

Lanzarini, G

Alfani, F

Cantarella, M

Gallifuoco, A

机构：

[1] Department of Industrial Chemistry and Materials, University of Bologna, 40136 Bologna

[2] Department of Biology, Defence and Biotechnology of Agroforestry, University of Basilicata, 85100 Potenza

[3] Department of Chemistry, Chemical Engineering and Materials, University of L'Aquila, 67040 Monteluco di Roio, AQ

来源：

PECTINS AND PECTINASES | 1996年 / 14卷

关键词：

D O I：

10.1016/S0921-0423(96)80274-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enzymatic degradation of pectin catalysed by Pectolyase Y23(RM) (Sheishin Co., Japan) was studied in ultrafiltration membrane reactors. The mixtures of degradation products were continuously ultrafiltrated in four cells connected in series and equipped with membrane of decreasing molecular weight cut-off. The different fractions recovered. The ratio of enzyme to substrate loading affected product distribution and particularly the yield of oligomers from 10 to 15 monomeric units. TLC analysis allowed to identify the composition of the samples at low molecular weight (below 0.5 KDa and 1 KDa). The rate of pectin degradation in batch reactors was not significantly affected by the presence of oligomers between 1 and 6 subunits.

引用

页码：439 / 449

页数：11

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