Comparison of methods for sample preparation of individual rat cerebrospinal fluid samples prior to two-dimensional polyacrylamide gel electrophoresis

被引：5

作者：

Garcia-Rodriguez, S ^{[1
]}

Castilla, SA ^{[1
]}

Machado, A ^{[1
]}

Ayala, A ^{[1
]}

机构：

[1] Univ Seville, Fac Farm, Dept Bioquim Bromatol & Toxicol, E-41012 Seville, Spain

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 22期

关键词：

rat cerebrospinal fluid; sample preparation; two-dimensional mapping;

D O I：

10.1023/B:BILE.0000003979.92664.11

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Different pre-treatment methods have been compared for two-dimensional mapping of individual rat cerebrospinal fluid samples based on acetone, trichloroacetic acid/acetone and methanol/acetone precipitation of proteins. Acetone precipitation following incubation with DTT gave the highest protein recovery (72%) and the largest number of protein spots (92 +/- 4) as well as minimizing the time taken.

引用

页码：1899 / 1903

页数：5

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