Mortalin imaging in normal and cancer cells with quantum dot immuno-conjugates

被引:86
作者
Kaul, Z [1 ]
Yaguchi, T [1 ]
Kaul, SC [1 ]
Hirano, T [1 ]
Wadhwa, R [1 ]
Taira, K [1 ]
机构
[1] Natl Inst Adv Ind Sci & Technol, AIST, Gene Funct Res Ctr, Tsukuba, Ibaraki 3058562, Japan
关键词
quantum dots; immunofluorescence; mortalin; visualization;
D O I
10.1038/sj.cr.7290194
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Quantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine, and have several predictive advantages. These include their i) broad absorption spectra allowing visualization with single light source, ii) exceptional photo-stability allowing long term studies and iii) narrow and symmetrical emission spectrum that is controlled by their size and material composition. These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source, their simultaneous resolution and visualization as different colors. At present there are only a few studies that have tested quantum dots in cellular imaging. We describe here the use of quantum dots in mortalin imaging of normal and cancer cells. Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable.
引用
收藏
页码:503 / 507
页数:5
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