Detection and tentative grouping of Strawberry crinkle virus isolates

被引:25
作者
Klerks, MM
Lindner, JL
Vaskova, D
Spak, J
Thompson, JR
Jelkmann, W
Schoen, CD
机构
[1] Plant Res Int BV, NL-6700 AA Wageningen, Netherlands
[2] Univ S Bohemia, Fac Biol Sci, Ceske Budejovice 37005, Czech Republic
[3] Acad Sci Czech Republ, Inst Plant Mol Biol, CR-37005 Ceske Budejovice, Czech Republic
[4] Biol Bundesanstalt Land & Forstwirtschaft, Inst Pflanzenschutz Obstbau, D-69221 Dossenheim, Germany
关键词
L protein; molecular beacon; NASBA; rhabdovirus; SCV; strawberry;
D O I
10.1023/B:EJPP.0000010134.06283.38
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A partial sequence of the putative polymerase (L) protein of Strawberry crinkle virus (SCV), genus Cytorhabdovirus, is described. The virus protein was found to be distantly related to the L protein of the rhabdoviruses Northern cereal mosaic virus, Rice yellow stunt virus and Sonchus yellow net virus. Moreover, a tentative grouping of SCV isolates is described, based on phylogenetic analysis of a region enclosing the GDN-motif within the RNA-dependent RNA polymerase gene. A sequence homology of 98% was found for each tentative group, and heterogeneity of at least 11% was observed between both groups. The tentative grouping did not appear to be related to symptomatology or geographical origin of the isolates. Nevertheless, the use of several reverse-transcription (RT)-PCR primer sets, all directed to different regions within the SCV genome, confirmed the presumptive classification into two groups, namely group I (isolates 1554, KG and Post), and group II (isolates 1553, Hb-A1, 37-1 and 37-2). Additionally, the detection of SCV isolates from herbaceous hosts and strawberry plant material was possible through use of a newly developed gel-based RT-PCR and a gel-free AmpliDet RNA assay. Both methods have the potential to provide rapid, sensitive and specific detection of SCV in in vitro propagation material.
引用
收藏
页码:45 / 52
页数:8
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