A Novel Alkaline Hemicellulosic Heteroxylan Isolated from Alfalfa (Medicago sativa L.) Stem and Its Thermal and Anti-inflammatory Properties

被引：24

作者：

Chen, Lei ^{[1
]}

Liu, Jie ^{[1
]}

Zhang, Yaqiong ^{[1
]}

Niu, Yuge ^{[1
]}

Dai, Bona ^{[2
]}

Yu, Liangli ^{[1
,3
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Inst Food & Nutraceut Sci, Shanghai 200240, Peoples R China

[2] Shanghai Jiao Tong Univ, Instrumental Anal Ctr, Shanghai 200240, Peoples R China

[3] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2015年 / 63卷 / 11期

基金：

中国博士后科学基金; 国家高技术研究发展计划(863计划);

关键词：

alfalfa polysaccharide; hemicellulosic heteroxylan; structural analysis; thermal decomposition; anti-inflammatory effect; CELL-WALL POLYSACCHARIDE; BROILER-CHICKENS; EXTRACTION; CHROMATOGRAPHY; PERFORMANCE; SUBSTANCES; COMPONENTS; KINETICS; PROTEIN; LUCERNE;

D O I：

10.1021/acs.jafc.5b00063

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

A novel hemicellulosic polysaccharide (ACAP) was purified from the cold alkali extraction of alfalfa stems and characterized as a heteroxylan with a weight-average molecular weight of 7.94 x 10(3) kDa and a radius of 58 nm. Structural analysis indicated that ACAP consisted of a 1,4-linked beta-d-Xylp backbone with 4-O-MeGlcpA and T-l-Araf substitutions at O-2 and O-3 positions, respectively. Transmission electron microscopy (TEM) examination revealed the entangled chain morphology of ACAP molecules. The evaluation of thermal degradation property revealed a primary decomposition temperature range of 238.8-314.0 degrees C with an apparent activation energy (E-a) and a pre-exponential factor (A) of 220.0 kJ/mol and 2.81 x 10(24)/s, respectively. ACAP also showed significant inhibitory activities on IL-1 beta, IL-6, and COX-2 gene expressions in cultured RAW 264.7 mouse macrophage cells. These results suggested the potential utilization of ACAP in functional foods and dietary supplement products.

引用

页码：2970 / 2978

页数：9

共 45 条

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