Evaluation of platelet function under high shear condition in the small-sized collagen bead column

被引:11
作者
Kaneko, M
Takafuta, T
Cuyun-Lira, O
Satoh, K
Arai, M
Yatomi, Y
Ozaki, Y
机构
[1] Univ Yamanashi, Fac Med, Dept Clin & Lab Med, Yamanashi, Japan
[2] Nishi Kobe Med Ctr, Dept Hematol & Clin Immunol, Kobe, Hyogo, Japan
[3] Tokyo Med Univ, Dept Lab Med, Tokyo, Japan
[4] Univ Tokyo, Dept Clin Lab Med, Grad Sch Med, Tokyo, Japan
来源
JOURNAL OF LABORATORY AND CLINICAL MEDICINE | 2005年 / 146卷 / 02期
关键词
D O I
10.1016/j.lab.2005.04.007
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
We previously reported that platelet retention rates as measured with collagen-coated bead columns (the conventional column) reflect the processes of platelet adhesion and aggregation under low shear stress, and that this system could serve as an easy-to-use platelet aggregometry. With this column, platelet glycoprotein (GP) VI and GPIIb/IIIa, but not the GPIb-von Willebrand factor (VWF) interaction, play major roles in platelet activation. To develop a system that can better reflect the GPIb-VWF interaction under high shear stress, we designed a column containing small-sized beads (125-212 mu m) coated with porcine collagen type I. As expected, the GPIb-VWF interaction played a crucial role in platelet retention rates at higher flow rates. Adenosine 5'-diphosphate, but not thromboxane A(2), appears to support platelet activation in this system. The platelet retention rates among healthy individuals with the new columns are in the range wider than the conventional columns, and this diversity could be attributed to the broad range of the VWF antigen and/or its activity. It is suggested that this new column can serve as an easy-to-use method for evaluating the VWF antigen levels and its activity and for monitoring patients with thrombotic or bleeding disorders related to the VWF-GPIb interaction.
引用
收藏
页码:64 / 75
页数:12
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