Production of ligninolytic enzymes by Phlebia floridensis

被引:38
作者
Arora, DS [1 ]
Gill, PK
机构
[1] Guru Nanak Dev Univ, Dept Microbiol, Microbial Technol Lab, Amritsar 143005, Punjab, India
[2] DAV Coll, Amritsar, Punjab, India
关键词
Laccase; lignin peroxidase; manganese peroxidase; white-rot fungi;
D O I
10.1007/s11274-004-7655-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];
摘要
The present work reports the production of laccase, lignin peroxidase and manganese peroxidase by the little studied white-rot fungus Phlebia floridensis under a variety of nutritional and physicochemical conditions. Among the different media and supplements the highest yields of laccase, lignin peroxidase and manganese peroxidase were recorded in the presence of sugarcane bagasse, wheat straw and rice straw, respectively. Laccase and manganese peroxidase activities were best expressed at a pH of 4.5 while lignin peroxidase was optimally active at a lower pH. Laccase proved to be much more thermostable as compared to the other two enzymes.
引用
收藏
页码:1021 / 1028
页数:8
相关论文
共 32 条
[1]
Decolourisation of diverse industrial dyes by some Phlebia spp. and their comparison with Phanerochaete chrysosporium [J].
Arora, DS ;
Chander, M .
JOURNAL OF BASIC MICROBIOLOGY, 2004, 44 (05) :331-338
[2]
LACCASE PRODUCTION AND WOOD DEGRADATION BY A WHITE-ROT FUNGUS DAEDALEA-FLAVIDA [J].
ARORA, DS ;
SANDHU, DK .
ENZYME AND MICROBIAL TECHNOLOGY, 1985, 7 (08) :405-408
[3]
Involvement of lignin peroxidase, manganese peroxidase and laccase in degradation and selective ligninolysis of wheat straw [J].
Arora, DS ;
Chander, M ;
Gill, PK .
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2002, 50 (02) :115-120
[4]
Comparison of two assay procedures for lignin peroxidase [J].
Arora, DS ;
Gill, PK .
ENZYME AND MICROBIAL TECHNOLOGY, 2001, 28 (7-8) :602-605
[5]
Effects of various media and supplements on laccase production by some white rot fungi [J].
Arora, DS ;
Gill, PK .
BIORESOURCE TECHNOLOGY, 2001, 77 (01) :89-91
[6]
Laccase production by some white rot fungi under different nutritional conditions [J].
Arora, DS ;
Gill, PK .
BIORESOURCE TECHNOLOGY, 2000, 73 (03) :283-285
[7]
Polycyclic aromatic hydrocarbon-degrading capabilities of Phanerochaete laevis HHB-1625 and its extracellular ligninolytic enzymes [J].
Bogan, BW ;
Lamar, RT .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1597-1603
[8]
COMPARATIVE STUDIES OF EXTRACELLULAR FUNGAL LACCASES [J].
BOLLAG, JM ;
LEONOWICZ, A .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (04) :849-854
[9]
MN(II) REGULATION OF LIGNIN PEROXIDASES AND MANGANESE-DEPENDENT PEROXIDASES FROM LIGNIN-DEGRADING WHITE ROT FUNGI [J].
BONNARME, P ;
JEFFRIES, TW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (01) :210-217
[10]
MANGANESE REGULATES EXPRESSION OF MANGANESE PEROXIDASE BY PHANEROCHAETE-CHRYSOSPORIUM [J].
BROWN, JA ;
GLENN, JK ;
GOLD, MH .
JOURNAL OF BACTERIOLOGY, 1990, 172 (06) :3125-3130