Vacuolar Glyphosate-Sequestration Correlates with Glyphosate Resistance in Ryegrass (Lolium spp.) from Australia, South America, and Europe: A 31P NMR Investigation

被引：105

作者：

Ge, Xia ^{[1
]}

d'Avignon, D. Andre ^{[1
]}

Ackerman, Joseph J. H. ^{[1
,2
,3
]}

Collavo, Alberto ^{[4
]}

Sattin, Maurizio ^{[4
]}

Ostrander, Elizabeth L. ^{[5
]}

Hall, Erin L. ^{[5
]}

Sammons, R. Douglas ^{[5
]}

Preston, Christopher ^{[6
]}

机构：

[1] Washington Univ, Dept Chem, St Louis, MO 63130 USA

[2] Washington Univ, Dept Internal Med, St Louis, MO 63130 USA

[3] Washington Univ, Dept Radiol, St Louis, MO 63130 USA

[4] Inst Agroenvironm & Forest Biol, I-35020 Legnaro, PD, Italy

[5] Monsanto Co, St Louis, MO 63167 USA

[6] Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2012年 / 60卷 / 05期

关键词：

glyphosate; glyphosate resistance; in vivo P-31 NMR; Lolium spp; glyphosate tonoplast transporter; RIGIDUM; TRANSLOCATION; POPULATIONS; INHERITANCE; MULTIFLORUM; MECHANISMS; HORSEWEED; PLANTS; WORLD;

D O I：

10.1021/jf203472s

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Lolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by P-31 NMR. Extents of glyphosate (i) resistance (LD50), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC50), and (iii) translocation were quantified for glyphosate-resistant (GR) and glyphosate-sensitive (GS) Lolium multiflorum Lam. variants from Chile and Brazil. For comparison, LD50 and IC50 data for Lolium rigidum Gaudin variants from Italy were also analyzed. All variants showed similar cellular uptake of glyphosate by P-31 NMR All GR variants showed glyphosate sequestration within the cell vacuole, whereas there was minimal or no vacuole sequestration in the GS variants. The extent of vacuole sequestration correlated qualitatively with the level of resistance. Previous P-31 NMR studies of horseweed (Conyza canadensis (L.) Cronquist) revealed that glyphosate sequestration imparted glyphosate resistance. Data presented herein suggest that glyphosate vacuolar sequestration is strongly contributing, if not the major contributing, resistance mechanism in ryegrass as well.

引用

页码：1243 / 1250

页数：8

共 38 条

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