Structure and regulation of OPR1 and OPR2, two closely related genes encoding 12-oxophytodienoic acid-10,11-reductases from Arabidopsis thaliana

被引：101

作者：

Biesgen, C ^{[1
]}

Weiler, EW ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Fak Biol, Lehrstuhl Pflanzenphysiol, D-44780 Bochum, Germany

来源：

PLANTA | 1999年 / 208卷 / 02期

关键词：

jasmonic acid; octadecanoid biosynthesis; 12-oxophytodienoic acid; 12-oxophytodienoic acid-10,11-reductase; promoter-GUS fusion;

D O I：

10.1007/s004250050545

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The genes of two closely related 12-oxophytodienoic acid reductases (EC 1.3.1.42), OPR1 and OPR2, were identified on a 7079-bp-long genomic fragment from Arabidopsis thaliana (L.) Heynh. The organization of these two genes was determined and putative cis elements were identified. Promoter-beta-glucuronidase (GUS) fusions expressed in transgenic Arabidopsis thaliana and Nicotiana tabacum L. plants revealed differences in OPR-promoter-driven GUS expression in flowers. While the OPR1 promoter directed GUS expression in young seeds, the OPR2 promoter directed pollen-specific expression. Both OPR1 and OPR2, were predominantly expressed in roots. Stress treatments, like local and systemic wounding, UV-C illumination and coldness, resulted in transient changes in steady-state OPR mRNA levels, but no concurrent changes in polypeptide level or enzyme activity were detected.

引用

页码：155 / 165

页数：11

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