Energy transfer-based lifetime sensing of chloride using a luminescent transition metal complex

被引:44
作者
Huber, C [1 ]
Werner, T [1 ]
Krause, C [1 ]
Klimant, I [1 ]
Wolfbeis, OS [1 ]
机构
[1] Univ Regensburg, Inst Analyt Chem Chemo & Biosensors, D-93040 Regensburg, Germany
关键词
energy transfer; decay time; chloride sensor;
D O I
10.1016/S0003-2670(98)00151-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A bulk membrane responsive to chloride has been developed containing a luminescent ruthenium complex placed in close proximity to the pH-sensitive absorber bromothymol blue (RTB), both contained in a plasticized poly(vinyl chloride) (PVC) membrane along with an anion-selective carrier. The response is based on the co-extraction of chloride along with protons from the aqueous sample into the PVC membrane and results in a large change in the absorbance of BTB. The absorption band of deprotonated BTB overlaps significantly with the emission band of the ruthenium complex so that radiationless energy transfer can take place and decay times are rather short (ca. 0.60 mu s). If, however, chloride is co-extracted into the bulk along with a proton, the dye is converted into the yellow phenolic form. Hence, no energy transfer occurs and decay times are in the order of 1 mu s. The signal changes can also be detected by measuring fluorescence intensity. The dynamic range can be adjusted via the sample pH and is from 30 to 180 mM chloride at pH 7.0 and thus covers the physiological range. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:143 / 151
页数:9
相关论文
共 20 条
  • [1] DETERMINATION OF OXYGEN CONCENTRATIONS BY LUMINESCENCE QUENCHING OF A POLYMER-IMMOBILIZED TRANSITION-METAL COMPLEX
    BACON, JR
    DEMAS, JN
    [J]. ANALYTICAL CHEMISTRY, 1987, 59 (23) : 2780 - 2785
  • [2] ANION-SELECTIVE MEMBRANE ELECTRODES BASED ON METALLOPORPHYRINS - THE INFLUENCE OF LIPOPHILIC ANIONIC AND CATIONIC SITES ON POTENTIOMETRIC SELECTIVITY
    BAKKER, E
    MALINOWSKA, E
    SCHILLER, RD
    MEYERHOFF, ME
    [J]. TALANTA, 1994, 41 (06) : 881 - 890
  • [3] BHATTI M, 1972, INORG NUCL CHEM LETT, V8, P135
  • [4] CHARLTON SC, 1982, CLIN CHEM, V28, P1857
  • [5] LUMINESCENT METAL-COMPLEXES .1. TRIS-CHELATES OF SUBSTITUTED 2,2'-BIPYRIDYLS WITH RUTHENIUM(II) AS DYES FOR LUMINESCENT SOLAR COLLECTORS
    COOK, MJ
    LEWIS, AP
    MCAULIFFE, GSG
    SKARDA, V
    THOMSON, AJ
    GLASPER, JL
    ROBBINS, DJ
    [J]. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1984, (08): : 1293 - 1301
  • [6] COTLOVE E, 1958, J LAB CLIN MED, V51, P461
  • [7] NOVEL TYPE OF ION-SELECTIVE FLUOROSENSOR BASED ON THE INNER FILTER EFFECT - AN OPTRODE FOR POTASSIUM
    HE, HR
    LI, H
    MOHR, G
    KOVACS, B
    WERNER, T
    WOLFBEIS, OS
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (02) : 123 - 127
  • [8] KOSCH U, UNPUB ANAL CHEM
  • [9] STRUCTURE ACTIVITY RELATIONSHIPS OF CHLORIDE-SENSITIVE FLUORESCENT INDICATORS FOR BIOLOGICAL APPLICATION
    KRAPF, R
    ILLSLEY, NP
    TSENG, HC
    VERKMAN, AS
    [J]. ANALYTICAL BIOCHEMISTRY, 1988, 169 (01) : 142 - 150
  • [10] Lakowicz J.R., 1983, PRINCIPLES FLUORESCE, DOI DOI 10.1007/978-1-4615-7658-7_9