TILLING without a plough: a new method with applications for reverse genetics

被引：101

作者：

Gilchrist, EJ ^{[1
]}

Haughn, GW ^{[1
]}

机构：

[1] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada

来源：

CURRENT OPINION IN PLANT BIOLOGY | 2005年 / 8卷 / 02期

关键词：

D O I：

10.1016/j.pbi.2005.01.004

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful reverse genetic technique that employs a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms. Its advantages over other reverse genetic techniques include its applicability to virtually any organism, its facility for high-throughput and its independence of genome size, reproductive system or generation time. TILLING is currently being used for the detection of both induced and natural variation in several plant species.

引用

页码：211 / 215

页数：5

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