Safety assessment of Cry1C protein from genetically modified rice according to the national standards of PR China for a new food resource

被引：56

作者：

Cao, Sishuo ^{[1
]}

He, Xiaoyun ^{[2
]}

Xu, Wentao ^{[1
,2
]}

Ran, Wenjun ^{[1
]}

Liang, Lixing ^{[1
]}

Luo, YunBo ^{[1
]}

Yuan, Yanfang ^{[1
]}

Zhang, Nan ^{[2
]}

Zhou, Xin ^{[2
]}

Huang, Kunlun ^{[1
,2
]}

机构：

[1] China Agr Univ, Lab Food Safety & Mol Biol, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

[2] Minist Agr, Supervis Inspect & Testing Ctr Genetically Modifi, Beijing 100083, Peoples R China

来源：

REGULATORY TOXICOLOGY AND PHARMACOLOGY | 2010年 / 58卷 / 03期

关键词：

Cry1C protein; Oral acute study; Safety assessment; Micronucleus assay; Sperm shape abnormality test; Ames test; GMO; THURINGIENSIS DELTA-ENDOTOXIN; BORDER MEMBRANE-VESICLES; INSECT RESISTANCE; MODIFIED CROPS; ALLERGENICITY; BINDING; TOXIN; GENE;

D O I：

10.1016/j.yrtph.2010.08.018

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The Cry1C protein produced in Escherichia coli was used for in vitro evaluation and animal studies to support the safety assessment of GM food or feed products containing the Cry1C protein. The Cry1C protein does not have any sequence homology with known allergens or toxins. Although the Cry1C protein was heat stable it was rapidly degraded in vitro with simulated gastric or intestinal fluids. It did not cause adverse effects in mice as administered by gavage at a high level dosage of 5 g (Cry1C protein)/kg body weight. The mutagenicity of this protein was evaluated according to the national standards of People's Republic of China (PR China) for a new food resource. In mutagenic tests, the Cry1C protein caused <4 micronucleated cells per 1000 cells, <16 sperm abnormalities per 1000 cells and was not associated with any increased mutations in the Ames test. Taken together, these data indicate that the Cry1C protein is not a potential allergen or toxin. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：474 / 481

页数：8

共 34 条

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