In vitro assessment of the immediate and prolonged antimicrobial action of chlorhexidine gel as an endodontic irrigant against Enterococcus faecalis

被引:84
作者
Dametto, FR [1 ]
Ferraz, CCR [1 ]
Paula, B [1 ]
Gomes, FD [1 ]
Zaia, AA [1 ]
Teixeira, FB [1 ]
de Souza, FJ [1 ]
机构
[1] Univ Estadual Campinas, Dept Restorat Dent, Sch Dent Piracicaba, BR-13414018 Piracicaba, SP, Brazil
来源
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY | 2005年 / 99卷 / 06期
基金
巴西圣保罗研究基金会;
关键词
D O I
10.1016/j.tripleo.2004.08.026
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective. The aim of this study was to assess in vitro the antimicrobial activity of 2% chlorhexidine gel against Enterococcus faecalis, comparing it to other endodontic irrigants (2% chlorhexidine liquid and 5.25% sodium hypochlorite). Study design. Eighty roots of human mandibular premolars were prepared by serial instrumentation technique, autoclaved, and contaminated for 7 days with E faecalis monocultures. The roots were then divided into 5 groups according to the irrigant substance used during the standardized biomechanical preparation. In order to evaluate the antimicrobial action of the irrigant substances, 3 microbial samples were taken: initial (before the biomechanical preparation); post-treatment (immediately after the biomechanical preparation), and final (7 days after the biomechanical preparation). The microbiological samples were plated to count the colony-forming units (CFU). Results. The 2% chlorhexidine gel and 2% chlorhexidine liquid significantly reduced the E faecalis CFU in the post-treatment and final microbiological samples. The 5.25% sodium hypochlorite also reduced the E faecalis CFU immediately after the root canal instrumentation, but it was not able to keep the root canal free of detectable E faecalis in the final sample. Conclusions. The 2% chlorhexidine gluconate (gel and liquid) antimicrobial ability was more effective than 5.25% sodium hypochlorite in keeping low CFU of E faecalis for 7 days after the biomechanical preparation.
引用
收藏
页码:768 / 772
页数:5
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