Structure of a polysaccharide from a Rhizobium species containing 2-deoxy-β-D-arabino-hexuronic acid

被引：57

作者：

Guentas, L

Pheulpin, P

Michaud, P

Heyraud, A

Gey, C

Courtois, B

Courtois, J

机构：

[1] Univ Picardie, Inst Univ Technol, Dept Gen Biol, Lab Polysaccharides Microbiens & Vegetaux, F-80025 Amiens, France

[2] CNRS, Ctr Rech Macromol Vegetales, UPR 5301, F-38041 Grenoble, France

来源：

CARBOHYDRATE RESEARCH | 2001年 / 332卷 / 02期

关键词：

extracellular polysaccharide; Rhizobium sp; structural analysis; 2-deoxy-arabino-D-hexuronic acid;

D O I：

10.1016/S0008-6215(01)00080-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the extracellular polysaccharide (EPS) produced by the Rhizobium sp. B strain isolated from atypical nodules on alfalfa has been determined using a combination of chemical and physical techniques (methylation analysis, high pH-anion exchange chromatography (HPAEC), mass spectrometry and 1-D and 2-D NMR spectroscopy). As opposed to the EPS from other strains of Rhizobium, the EPS from the sp. B strain contains D-Glc together with L-Rha and 2-deoxy-D-arabino -hexuronic acid. It is a polymer of a repeating unit having the following structure: --> 4)-beta -D-Glcp -(1 --> 4)-alpha -L-Rhap-(1 --> 3)-beta -D-Glcp -(1 --> 4)-2-deoxy-beta -D-GlcpA-(l --> The polysaccharide also contains 0.6 O-acetyl groups per sugar which have not been located. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：167 / 173

页数：7

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