Interaction of spermine with a signal transduction pathway involving phospholipase C, during the growth of Catharanthus roseus transformed roots

被引：16

作者：

Echevarría-Machado, I ^{[1
]}

Ku-González, A ^{[1
]}

Loyola-Vargas, VM ^{[1
]}

Hernández-Sotomayor, SMT ^{[1
]}

机构：

[1] Ctr Invest Cient Yucatan, Unidad Bioquim & Biol Mol Plantas, Merida 97200, Yucatan, Mexico

来源：

PHYSIOLOGIA PLANTARUM | 2004年 / 120卷 / 01期

关键词：

D O I：

10.1111/j.0031-9317.2004.0212.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In Cantharanthus roseus transformed roots, the application of methylglyoxal bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC; EC 4.1.1.50), inhibited the root growth in a dose-dependent manner with a DL50 of about 300 mum. Spermidine and spermine (Spm) levels and SAMDC and phospholipase C (PLC; EC 3.1.4.3) activities were reduced in the presence of the inhibitor. The inhibition was reversed by the addition of Spm. Radioactivity from [C-14]Spm was detected in an immunoprecipitated fraction with an antibody anti-PLC-delta. To our knowledge, this is the first direct evidence that demonstrates an interaction of Spm with the signal transduction cascade phosphoinositide-Ca2+.

引用

页码：140 / 151

页数：12

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