Enzyme engineering: rational redesign versus directed evolution

被引：104

作者：

Chen, RD ^{[1
]}

机构：

[1] Univ Saskatchewan, Coll Med, Dept Biochem, Saskatoon, SK S7N 5E5, Canada

来源：

TRENDS IN BIOTECHNOLOGY | 2001年 / 19卷 / 01期

关键词：

D O I：

10.1016/S0167-7799(00)01522-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enzyme engineering is undergoing the most profound and exciting transformation in its history(1). It promises unprecedented expansion in the scope and applications of modified or improved enzymes with desired physical and catalytic properties. Two complementary strategies are currently available: rational redesign(2-5) and directed evolution(6,7). Although both approaches have met with great success, each has limitations. In this article, the perspectives for these enzyme-engineering strategies are discussed briefly.

引用

页码：13 / 14

页数：2

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