Mutual stabilization of the XcpZ and XcpY components of the secretory apparatus in Pseudomonas aeruginosa

被引：61

作者：

Michel, G ^{[1
]}

Bleves, S ^{[1
]}

Ball, G ^{[1
]}

Lazdunski, A ^{[1
]}

Filloux, A ^{[1
]}

机构：

[1] CNRS, IBSM, UPR9027, Lab Ingn Syst Macromol, F-13402 Marseille 20, France

来源：

MICROBIOLOGY-SGM | 1998年 / 144卷

关键词：

general secretory pathway; protein secretion; protein-protein interaction; protein stability; Pseudomonas aeruginosa;

D O I：

10.1099/00221287-144-12-3379

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Protein secretion in Gram-negative bacteria is often dependent on the general secretory pathway (GSP). In Pseodomonas aeruginosa, this system requires at least 12 Xcp (Gsp) proteins, which are proposed to constitute a multiprotein complex localized in the bacterial envelope. Hitherto, little was known about the mutual interactions between Xcp proteins. In this study, mutants affected in the xcpZ gene encoding a bitopic inner-membrane protein were analysed to investigate the role of this protein in the architecture of the secretory machinery. The absence of XcpZ resulted in a decreased amount of XcpY. Reciprocally, XcpZ was not detectable in a xcpY mutant, demonstrating a mutual stabilization of these two proteins. These results strongly suggest that XcpZ and XcpY interact within the functional secretory apparatus.

引用

页码：3379 / 3386

页数：8

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